NIGERIAN JOURNAL OF SCIENCE AND ENVIRONMENT
Journal of the Faculties of Science and Agriculture, Delta State University, Abraka, Nigeria
ISSN: 1119-9008
DOI: 10.5987/UJ-NJSE
Email: njse@universityjournals.org
PARTIAL PURIFICATION AND CHARACTERIZATION OF β-DGALACTOSIDASE FROM PLANTAIN FRUIT (Musa paradisiaca L.)
DOI: 10.5987/UJ-NJSE.16.039.1 | Article Number: E69F3C5 | Vol.11 (1) - September 2012
Authors: Agoreyo B.O and Golden K.D
Keywords: Musa paradisiaca, plantain, galactosidase/β-D-Galactosidase in plantain
β-D-Galactosidase (EC 3.2.1.23) from ripe plantain fruit was partially purified and characterized. Purification was carried out using ammonium sulphate precipitation and gel-filtration on Sephadex G25-150 and G-25M. The enzyme displayed activity against p-nitrophenyl-β-D-galactopyranoside, with a Km of 1 mM. It was inhibited by galactose and mercuric chloride. Galactose was a noncompetitive inhibitor, while mercuric chloride was an uncompetitive inhibitor.
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