NIGERIAN JOURNAL OF SCIENCE AND ENVIRONMENT
Journal of the Faculties of Science and Agriculture, Delta State University, Abraka, Nigeria
ISSN: 1119-9008
DOI: 10.5987/UJ-NJSE
Email: njse@universityjournals.org
ANTIMICROBIAL ACTIVITY OF THE CRUDE POLYSACCHARIDE EXTRACTS OF THE EDIBLE MUSHROOM PLEUROTUS OSTREATUS (JACQ FR.) KUMM BEFORE AND AFTER BOILING.
DOI: 10.5987/UJ-NJSE.17.088.3 | Article Number: CEBC2B9 | Vol.10 (3) - December 2011
Authors: Emoghene, A. O. and Onwudinjo C. J.
Keywords: antimicrobial, polysaccharide, extract, edible mushroom, clinical isolates
The antimicrobial effects of the crude polysaccharide extract of the mushroom, Pleurotus ostreatus on some clinical isolates were investigated using the agar well diffusion method. Ethanol polysaccharide extracts of raw P. ostreatus showed sizeable inhibition zones of 15mm, 15mm, 16mm, 18mm and 12mm on Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae and Candida albicans respectively. The ethanol extract of boiled P. ostreatus also showed sizeable inhibition zones of 11mm, 12mm and 6mm on Escherichia coli, Klebsiella pneumoniae and Candida albicans respectively. The aqueous extracts of the raw P. ostreatus showed inhibition zone of 5mm against Pseudomonas aeruginosa while aqueous extract of boiled P. ostreatus showed no observable zones of inhibition against the test organisms. The minimum inhibition concentration determined by the agar well diffusion technique for the ethanol extracts of raw P. ostreatus were 25mg/ml, 50mg/ml, 50mg/ml, 50mg/ml and 25mg/ml for Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae and Candida albicans respectively while minimum inhibition concentration for the ethanol extract of boiled P. ostreatus were 25mg/ml, 50mg/ml, 50mg/ml, 50mg/ml and 100mg/ml for Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Klebsiella pneumoniae and Candida albicans respectively. The outcome of this study showed that antimicrobial activity using extracts of P. ostreatus has potential.
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