NIGERIAN JOURNAL OF SCIENCE AND ENVIRONMENT
Journal of the Faculties of Science and Agriculture, Delta State University, Abraka, Nigeria
ISSN: 1119-9008
DOI: 10.5987/UJ-NJSE
Email: njse@universityjournals.org
THE EFFECT OF SARCOCEPHALUS LATIFOLIUS AND DANIELLA OLIVERI ROOTS EXTRACT ON RENAL OXIDATIVE STRESS IN DIABETIC RATS
DOI: 10.5987/UJ-NJSE.17.075.3 | Article Number: C702CE2 | Vol.10 (3) - December 2011
Authors: Ojiako O. A. , Iheanacho K. M. E. , Joshua P. E. , Shoyinka S. V. , Nwodo O. F. C. and Iwueke A. V.
Sarcocephalus latifolius and Daniella oliveri are tropical plants whose roots are used in combination for the management of diabetes mellitus among other medicinal uses. This study investigated the effect of the aqueous extract of these roots (SDAE) on renal lipid peroxidation products (MDAs) and oxidative stress in non-diabetic control and alloxan-induced diabetic rats. 20 male wistar rats were induced in this study. Moderate diabetes mellitus was induced with alloxan (65mg/kg body weight) in 15 rats; these animals were further grouped into three (B,C & D). Group B was left untreated, Group C and D were treated intragastrically with SDAE (250mg/kg) and glibenclamide (5mg/kg) respectively. Treatments were administered twice daily for three weeks. MDA significantly (p<0.05) increased, while the reduced glutathione (GSH) concentration de- creased in the kidney of untreated diabetic rats. This was accompanied by a significant increase (p<0.05) in both catalase and superoxide dismutase activities. The diabetes-induced increase in MDA was non-significantly (p>0.05) reduced by treatment with the extract. The result also indi- cated that the aqueous extract normalized the GSH concentration in the diabetic kidney. The ex- tract caused a non-significant decreased in the SOD activity and almost normalized the catalase activity in the diabetic kidney. Renal histopathological changes induced by diabetes were im- proved by the extract to an extent. The current study suggests that the extract is effective in pre- venting oxidative/peroxidative stress and also ameliorates endogenous antioxidant enzyme activi- ties in the diabetic kidneys; confirming its ethnopharmacological use for the management of diabe- tes and diabetes-induced complications.
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