NIGERIAN JOURNAL OF SCIENCE AND ENVIRONMENT
Journal of the Faculties of Science and Agriculture, Delta State University, Abraka, Nigeria
ISSN: 1119-9008
DOI: 10.5987/UJ-NJSE
Email: njse@universityjournals.org
COMPARISON OF RAPID DIAGNOSTIC TEST (RDT), POLYMERASE CHAIN REACTION (PCR) AND MICROSCOPY METHODS IN THE DIAGNOSIS OF MALARIA AMONG AIRPORT WORKERS IN LAGOS
DOI: 10.5987/UJ-NJSE.16.053.1 | Article Number: 9A2C5E4 | Vol.14 (1) - July 2016
Authors: Kanyi O. I , Ajayi M. B , Afocha E. E , Iwalokun B and Ezeugwu S. M. C
Keywords: Malaria diagnostic, RDT, PCR, microscopy, Lagos
Malaria is one of the leading causes of death in Africa, and it is highly endemic in Nigeria. It is responsible for about 70% of out-patient attendance at secondary health care facilities in Lagos State. This study assessed the diagnostic performance of three malaria detection methods among Nigerian airport workers. 268 blood samples were collected from febrile and non-febrile Airport workers in Lagos, and processed using three diagnostic methods: thick and thin Giemsa blood stained microscopy method for parasite species identification and count; SD-Bioline PfLDH/panLDH rapid diagnostic test (RDT) device; and Polymerase Chain Reaction (PCR) amplification as a two step procedure using dried blood spot (DBS). A total prevalence of 2.6% was detected by microscopy, 4.1% by RDT and 4.9% by PCR. Microscopy parasite density is 22 to 5.837/µl at prevalence rate of 4.9%. The species identified were 69% Plasmodium falciparum and 31% Plasmodium malariae, while P. falciparum and P. malariae co-infections were observed. The performance evaluation shows PCR having 100% sensitivity while microscopy and RDT had 53.8 and 84.6%, respectively. The negative predictive values are 97.8, 99.3 and 100% for microscopy, RDT and PCR respectively. The result of the PCR and RDT methods indicated that the gold standard method (microscopy) is less sensitive because additional 2.3% positive cases were detected by the PCR method
Ademowo, O.G., Falusi, A.G. and Mewoyeka, O.O. (1995). Prevalence of asymptomatic parasitaemia in an urban and rural community in Southwestern Nigeria. Central African journal of Medicine. 42(12): 411-412;
Agboola, T.F., Ajayi, M.B., Adeleke, M. A. and Gyang, P.V. (2010). Prevalence of malaria parasite among donors in Lagos University Teaching Hospital. Annals of Biological Research. 1:72-75;
Agomo, O. Chimere, Oyibo A. Wellington, Anorlu I. Rose, and Agomo U. P. (2009). Prevalence of Malaria in Pregnant Women in Lagos, South-west Nigeria. The Korean Journal of Parasitology. 47(2): 179-183.
Anthony, M. (2002). Rapid Diagnostic Tests for Malaria Parasites. Clinical Microbiological Reviews. 15 :1 66-78.
Arsène, R., Harintsoa, R., Jeanne-Aimée, V., Rogelin, R., Martial, J., Rabenja, R., Jean De Dieu, M., Rakotomanga, D. M., Pascal M. and Didier M (2012). Management of uncomplicated malaria in febrile under five-year-old children by community health workers in Madagascar: reliability of malaria rapid diagnostic tests. Malaria Journal. 11:85.
Bereczky, S., Andreas, M., Pedrogh, J and Ama, F. (2005). Short Report: Rapid DNA extraction from archive blood spots on filter paper for Genotyping of P.falciparum. American Journal of tropical Medicine and Hygiene. 77(3): 249-251;
Brian, M. G., David, A. F., Dennis, E. K, Stefan, H.I. K., Pedro, L. A., Frank, H. C. and Patrick, E. D. (2008). Malaria: Progress, Perils and Prospects for eradication. Journal of Clinical Investigation. 118(4): 1266-1276.
Centers for Disease Control and Prevention (CDC). (2014). Malaria Worldwide-Impact of Malaria. Global Health –Division of Parasitic Diseases and malaria..
Dougnou, V., Bankole, H.S., Hounmanou, Y.M.G., Echebiri, S., Atchade, P. and Mohammed, J. (2015). Comparative study of Malaria Prevalence among travelers in Nigeria (West Africa) using slide microscopy and a rapid Diagnosis test. Journal of Parasitology Research. (108707): 4-4; 2015.
Erhabor, O., Babatunde, S. and Uko, K.E. (2006). Some haematological parameters in Plasmodial parasitized HIV-infected Nigerians. Nigerian Journal of Medicine. 15(1): 52-55.
Federal Ministry of Health (FMoH). (2005). National Malaria and Vector Control Division Abuja-Nigeria. National Antimalarial treatment Policy.
Georges, S. (1996). Detection and Identification of the four malaria parasitic species infecting humans by PCR amplification. Methods in molecular biology (Cifton; N.J.) 50:263-91
Hopkins, H., Kambale, W., Kamya, M.R., Staedke, S.G., Dorsey, G. and Rosenthal, P. (2007). Comparison of HRP-2 and pLDH-based rapid diagnostic tests for malaria with longitudinal follow-up in Kampala, Uganda. American Journal for Tropical Medicine & Hygiene. 76: 1092.
Igbeneghu, C. and Odaibo, B. A. (2012). Plasmodium species among the inhabitants of Iwo community, Southwestern Nigeria. American-Eurasian Journal of Scientific Research 7(3): 118-122,
Iqbal, J., Parsotam, R. H., Ali, S. and Abdul A. A. (2001). Diagnosis of imported malaria by Plasmodium lactate dehydrogenase (pLDH) and Histidine-rich protein-2 PfHRP-2) – BASED Immunocapture assays. American Journal of Tropical Medicine and Hygiene. 64(1,2):20-23.
Isah, Z., Abdulrahaman, Y., Augustine, N. O., Ishaku, E. Y., Osaro, E., Abdullah, S. M. and Kufre, E. U. (2013). Prevalence of malaria Parasitaemia and methaemoglobin levels among blood donors in Sokoto, Nigeria. Internal Medicine Inside, 1:4.
Jamshaid, I., Ali S., Parsotan, R H. and Rashed, A. (1999). Comparison of the OptiMAL PCR for the diagnosis of malaria in immigrants. Journal of Clinical Microbiology. 37(11): 3644-3646.
Kassberger, F., Birkenmaier, A., Khattab, A., Kremsner, P.G. and Klinkert, M.Q. (2002). PCR typing of Plasmodium falciparum in matched peripheral, placental and umbilical cord blood. Journal of Parasitological Research. 88: 1073-1079.
Lagos State ministry of Health (LSMoH). (2015). Malaria Control program.
Marielle, K.B.A., Genis, E.I.C., Modeste, M.M., Eric, K., Pierre, B.M. and Maryvome, K. (2003). Prevalence of Plasmodium falciparum infection in pregnant women in Gabon. Malaria Journal.2:1-17.
May, J., Mockenhaupt, F.P., Ademowo, O.G., Falusi, A.G., Olumese, P.E., Bienzle, U. and Meyer, C.G. (1999). High rate of mixed and subpatent malarial infections in Southwest Nigeria. American Journal of Tropical Medicine and Hygiene. 61(2): 339-343;
Mekonnen, S. K., Abraham, A., Girmay, M., Nega, B. and Thirumalaisamy, P. V. (2014). Re-evaluation of microscopy confirmed Plasmodium falciparum and Plasmodium vivax malaria by nested PCR detection in Southern Ethiopia. Malaria Journal, 13:48
Miguel, R.B., Coura, J.R., Samudio, F. and Suarez-Mutis, M.C. (2013). Evaluation of three different DNA Extraction methods from blood samples collected in dried filter paper in Plasmodium Subpatent infections from the Amazon region in Brazil. Revista do Instituto de Medicina Tropical de São Paulo. 55(3):205-208.
Molineaux, L., Storey, J., Cohen, J.E. and Thomas, A. A. (1980). Longitudinal study of human malaria in the West Africa Savanna in the absence of control measures: relationships between different Plasmodium species, in particular P.falciparum and P.malariae. American Journal of Tropical Medicine and Hygiene. 29:725-737.
Nandwani, S., Mathur, M., Rawat, S. (2003). Evaluation of the direct acridine orange staining method and Q.B.C. test for diagnosis of malaria in Delhi, India. Journal of Communicable diseases 35 (4):278-282.
Nigeria Malaria Indicator Survey. (2010). Final Report.
Summiya, N. (2009). Laboratory Diagnosis of Malaria. Pakistan Antimicrobial Resistance Network.
Uko, E.K., Emeribe, A.O. and Ejezie, G.C. (998). Malaria Infection of the placenta and neo-natal low birth weight in Calabar. Journal of Medical Laboratory Science. 7(2): 7-10.
Umaru, M. L. and Uyaiabasi, G. N. (2015). Prevalence of Malaria in Patients attending the General Hospital Markafi, Markafi Kaduna State, North-western Nigeria. American Journal of Infectious Diseases and Microbiology. 3(1): 1-5.
World Health Organisation. (2013). WHO Microscopy, Malaria, Areas of work, Diagnostic testing.
World Health Organisation. (2013). World Malaria Report. WHO Global Malaria Programme. WHO Malaria Fact Sheet. No 94, Dec. 2013.