Journal of the Faculties of Science and Agriculture, Delta State University, Abraka, Nigeria

ISSN: 1119-9008
DOI: 10.5987/UJ-NJSE


DOI: 10.5987/UJ-NJSE.17.131.1   |   Article Number: 400F07416   |   Vol.15 (1) - November 2017

Author:  Okoro O. Israel

Keywords: Ethulia conyzoides, antioxidant activity, radical scavenging activity (DPPH), reducing power activity, ferric thiocyanate (FTC), thiobarbituric acid (TBA).

The present study was aimed to examine the in vitro antioxidant potential of Ethulia conyzoides aerial parts extracts. Different parts of E. conyzoides have been employed in traditional medicine for treating several diseases. Solvents of different polarity (petroleum ether, acetone and ethanol) were used to prepare extracts of the plant for determining their in vitro antioxidant activities using diverse in vitro assays. The antioxidant activity was measured by using 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, ferric thiocyanate (FTC), reducing power and thiobarbituric acid (TBA) assay methods. Here, ascorbic acid (ASA) was used as standard antioxidant. Results obtained from the study shows that all extracts of the plant have significant scavenging activity against DPPH (45.85% for acetone, 42.00% for ethanol and 37.85% for petroleum ether extracts at 2 mg/ml each). Furthermore, the three extracts of E. conyzoides also demonstrated significant antioxidant activity in other in vitro assays with the acetone extract being the most active. Hence the present antioxidant studies of E. conyzoides, will be beneficial in the synthesis/ preparation of new drugs of pharmaceutical importance, especially its acetone extract.

Aiyegoro, O. A. and Okoh, A. I (2010). “Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC,” BMC Complementary and Alternative Medicine, 10:21.

Aliyu, A.B., Ibrahim, H., Ibrahim, M.A., Musa, A.M., Lawal, A.Y., Oshanimi, J.A., Usman, M., Abdulkadir, I., Oyewale, A.O. and  Amupitan, J.O (2012). Free radical scavenging and total antioxidant capacity of methanol extract of Ethulia conyzoides growing in Nigeria. Romanian Biotechnological Letters; 17(4): 7458-7465.

Bhuiyan, M.A.R., Hoque, M.Z. and Hossain, S.J (2009). Free Radical Scavenging Activities of Zizyphus mauritiana. World J. Agricul. Sci., 5: 318-322.

Burkill, H. M (1985). The Useful Plants of West Tropical Africa. (vol. 1): families A-D. The Royal Botanical Garden, Kews; p.960.

Dorman, H.J., Kosar, M., Kahlos, K., Holm, Y., and Hiltunen, R (2003). Antioxidant properties and composition of aqueous extracts from Mentha species, hybrids, varieties, and cultivars. J. Agricul. Food Chem., 51: 4563–4569.

Droge, W (2002). “Free radicals in the physiological control of cell function,” Physiol. Rev., 82: 47–95.

Duh, P.D (1998). Antioxidant activity of burdock (Arctium lappa Linne.): Its scavenging effect on free-radical and active oxygen. J. Am. Oil Chem. Soc. 75: 455-461.

Duh, P.D., Du, P.C. and Yen, G.C. (1999). Action of methanolic extract of mung beans hulls as inhibitors of lipid peroxidation and non-lipid oxidative damage. Food and Chemical Toxicology, 37: 1055-1061.

El-Bassuony, A (2009). Antibacterial activity of two new monoterpene coumarins from Ethulia conzoides. J.  Pharm. Res., 2(4): 582-584.

Gordon, M.H. (1990).  The mechanism of antioxidant action in vitro. In Food antioxidants. Edited by Hudson BJ. London: Elsevier Applied Science, pp. 1–18.

Gulcin, I (2012). Antioxidant activity of food constituents: an overview. Arch. Toxicol., 86:345–391.

Jayaprakash, G.K., and Rao, L.J (2000). Phenolic constituents from lichen Parmontrema stuppeum. Food control, 56: 1018-1022.

Kady, M.M., Brimer, L., Furu, P., Lemmich, E., Neilsen, H.M., Thiilborg, S.T., Thastrup, O., Chritensen, S.B  (1992). The molluscicidal activity of coumarins from  Ethulia conyzoides and of dicumarol. Planta Medica, 58(4): 334-337.

Krishnaiah, D., Sarbatly, R. and Nithyanandam, R.R. (2011). A review of the antioxidant potential of medicinal plant species. Food and Bioproducts Processing, 89:217–233.

Lin, C.C., and Huang, P.C (2002). Antioxidant and hepatoprotective effects of Acathopanax senticosus. Phytotherapy Research 14: 489–494.

Liyana-Pathirana, C.M. and Shahidi, F (2005). Antioxidant activity of commercial soft and hard wheat (Triticum aestivum L.) as affected by gastric pH conditions. J. Agricul. Food Chem. 53: 2433–2440.

Mahmoud, Z.F., Sarg, T.M., Amer, M.E., and Khafagy, S.M. (1983). Anthelmintic coumarin from Ethulian conyzoides var. gracilis Asch. and Schweinf. Pharmazie; 38(7): 486-487.

Maqsood S., Singh, P., Samoon, M.H., and Balange, A. K (2010). Effect of dietary chitosan on non-specific immune response and growth of Cyprinus carpio challenged with Aeromonas hydrophila. International Aquatic Research, 2:77–85.

Nunes, P.X., Silva, S.F., Guedes, R.J. and Almeida, S (2012). Biological Oxidations and Antioxidant Activity of Natural Products. Croatia: Tech publisher

Okoro, I.O., Umar, I. A., Atawodi, S.E. and Anigo, K.M. (2014). Comparative antihyperglycemic effect of petroleum ether, acetone, ethanol and aqueous extracts of Cleome rutidosperma DC and Senecio biafrae (Oliv. & Hiern) in streptozotocin-induced diabetic mice. Brit. J. Pharmacol. Toxicol. 5(3): 115-124.

Osawa, T. and Namiki, T (1981). A novel type of antioxidant isolated from leaf wax of Eucalyptus leaves. J. Agricul. Food Chem. 45: 735-739.

Ottolenghi, A (1959). 2-Thiobarbituric acid (TBA) method. Archives of Biochemistry and Biophysics. 77: 355-359.

Oyaizu, M (1986). Studies on product of browning reaction prepared from glucose amine. Japanese Journal of Nutrition., 44: 307-315.

Perry, E.K., Pickering, A.T., Wang, W.W., Houghton, P.J. and Perru, N.S (1999). Medicinal plants and Alzheimer’s disease: from ethnobotany to phytotherapy. J. Pharm. Pharmacol., 51:527–534.

Repetto, M.G., and Llesuy, S.F (2002). Antioxidant properties of natural compounds used in popular medicine for gastric ulcers. Braz. J. Med. Biol. Res., 35:523–534.

Roy, H. B (1994). Free Radical Damage and its control, Elsevier Science B.V. Netherlands. p.125.

Sen, S., Chakraborty, R., Sridhar, C., Reddy, Y. S. R., and De, B (2010). “Free radicals, antioxidants, diseases and phytomedicines: current status and future prospect,” Inter. J. Pharmaceut. Sci. Rev. Res., 3(1): 91–100.

Sharma, S., Nagpal, A., and Vig, A.P (2012).“Genoprotective potential of Brassica juncea (L.) Czern. against mercury-induced genotoxicity in Alliumcepa L,” Turk. J. Biol., 36: 622–629.

Shukla, V.S., Dutta, S.C., Baruah, R.N., Sharma, R.P., Thyagarajan, G., Herz, W., Kumar, N., Watanabe, K., and Blount, J.F (1982). New 5-methylcoumarins from Ethulia conyzoides. Phytochemistry; 21(7): 1725-1731.

Sowemimo, A., Van de Venter, M., Baatjies,  L. and Koekemoer, T (2009).  Cytotoxic Activity of Selected Nigerian Plants, Afr. J. Tradit. Complem. Alter. Med., 6(4): 526 – 528.

Tara, C., Anil B., Bhupendra, K. K., Pawank, B., Sanjay, S., and Rajesh, V (2012). In vitro antioxidant activity of alcoholic extract of seed of Cucumis callosus (Rottl.) cogn. Am. J. Pharmtech. Res., 2(3): 2249-3387.

Zengin, G., Cakmak, Y.S., Guler, G.O., and Aktumsek, A (2011). Antioxidant properties of methanolic extract and fatty acid composition of Centaurea urvillei DC. subsp. hayekiana Wagenitz. Records of Natural Products, 5:123–132.

Wagner, W. L., Herbst, D. R., and Sohmer, S. H. (1999). Manual of the Flowering Plants of Hiwaii, vol. 2, Bishop Museum Press, University of Hiwaii, Honolulu, Hawaii, USA.

Yildirim, A., Mavi, A., Oktay, M., Kara, A.A., Algur, O.F., and Bilaloglu, V (2000). Comparison of antioxidant and antimicrobial activities of tilia (Tilia arentea Desf. Ex. D.C.) sage (Salvia triloba L.) and black tea (Camellia sinensis L.) extracts. J. Agricul. Food Chem., 48(10): 5030-5034.